山羊不同组织及不同发育时期骨骼肌内参基因的表达稳定性分析

doi:10.11843/j.issn.0366-6964.2014.08.004

Abstract

Abstract:

To screen the reference genes of stable expression in different tissues，skeletal muscle of different development periods and skeletal muscle satellite cells (SMSCs) in goat，the expression levels of 9 reference genes of ACTIN，GAPDH，TOP2β，YWHAZ，SDHA，PGK1，RP2，RPL4 and HPRT1 mRNA were detected in different tissues (heart，liver，spleen，lung，kidney，brain，small intestine，large intestine,longissimus dorsi，triceps brachii and semimembranosus ) of 3-day-old postnatal kids，and the longissimus dorsi，triceps brachii and semimembranosus of 3，30，60，90 and 120 days old goat and the SMSCs cultured for 1，3，4 and 7 d.The data of expression levels were analyzed using the geNorm software.The results showed that the expressive stability of nine reference genes from high to low were HPRT1，RP2，TOP2β，SDHA，GAPDH，ACTIN，YWHAZ，PGK1，RPL4，and the 2 genes (HPRT1 and RP2) were needed to normalize the qRT-PCR data in different tissues of neonatal goat kids.Meanwhile，the stability of 9 references genes decreased as following: YWHAZ，ACTIN，HPRT1，RPL4，TOP2β，SDHA，PGK1，GAPDH，RP2，and the first 3 genes (YWHAZ，ACTIN and HPRT1) were suggested to accurately measure the true expression levels of target genes during the skeletal muscle of different development stages.Additionally，their expressive stability rank were PGK1，SDHA，ACTIN，RPL4，GAPDH，TOP2β，YWHAZ，RP2，HPRT1 in SMSCs，and 3 genes (PGK1，SDHA and ACTIN) were recommend as standardized factors.Here，the expressive stability of 9 reference genes and suitable genes were confirmed in different tissues，the skeletal muscle of different development periods and the SMSCs in goat.This will be beneficial to the research about the expression and function of gene at mRNA level.

CLC Number:

S827
Q786

CHEN Li，ZHAO Wei，ZHAN Si-yuan，LI Dan，LI Li，ZHONG Tao，WANG Lin-jie，ZHANG Hong-ping. The Expression Stability Analysis of Reference Genes in the Different Tissues and Skeletal Muscle of Different Development Periods in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1228-1236.

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The Expression Stability Analysis of Reference Genes in the Different Tissues and Skeletal Muscle of Different Development Periods in Goat

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